Amazon confirmed the closure following a regular assessment of its tools and services.
The platform was famously described by founder Jeff Bezos as "artificial artificial intelligence" because it used human labor to power digital processes.
Its closure marks the end of an era for a pioneer in the gig economy that once supported over 500,000 workers.
The service has recently faced increased competition from specialized data startups like Scale AI and Prolific, which recruit workers specifically to train modern AI models.
Additionally, a 2023 study found that nearly half of Mechanical Turk workers were using AI themselves to complete tasks, complicating the platform's original purpose of providing human-verified data.
While Amazon stopped accepting new customers last month, current users and workers have until late 2026 to transition away from the platform.
The shutdown affects a diverse range of companies, including those in the insurance and travel sectors, that still rely on the service for data processing.
For the remaining workforce, many of whom utilize the platform for supplemental or full-time remote income, the closure necessitates a shift to alternative data annotation services to maintain their earnings.